基于在pH8．5碱性介质中阿仑膦酸钠经4-氯-7-硝基苯并-2-氧杂-1，3-二唑（NBF-C1）衍生化后生成一种黄色荧光物质，该荧光物质经激发光照射产生荧光，据此提出了荧光光谱法测定中阿仑膦酸钠含量的方法。在激发波长457nm、发射波长532nm处，反应体系荧光强度与阿仑膦酸钠的质量浓度在0．02～1.0mg·L^-1范围内呈线性关系。方法用于阿仑膦酸钠片剂的分析，测得加标回收率为97．1％～113％，相对标准偏差（n=6）为0．06％～0．14％。%Based on the derivation reaction between sodium alendronate and 4-chloro-7-nitrobenzofurazan (NBF-CI) to give a yellow fluorescent product in a medium of pH 8. 5 and on the fluorescence property of the derivate as shown by its fluorescence spectrum at the wavelengths of 457 nm (λex) and 532 nm (λem), a fluorophotometric method for determination of sodium alendronate was proposed. Linear relationship was found between values of fluorescence intensity and mass concentration of sodium alendronate in the range of 0. 02- 1.0 mg ·L^-1. The proposed method was used in the analysis of samples of sodium alendronate tablets, giving values of recovery ranged from 97. 1%-113%, and RSD's (n=6) ranged from 0. 06%-0. 14%.
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