维生素B2在聚茜素红S修饰碳糊电极上的电化学行为及其应用

摘要

采用循环伏安法在碳糊电极(CPE)上制备了聚茜素红S薄膜修饰碳糊电极(ARS/CPE),运用循环伏安法和差分脉冲伏安法研究了维生素B2在该修饰电极上的电化学行为.结果表明:在pH4.6的0.1 mol·L-1乙酸-乙酸钠缓冲溶液中,该修饰电极对维生素B2的氧化还原反应具有良好的电催化作用,扩散系数(D)为3.26×10-5cm2·S-1,电荷转移系数(α)为0.817 4.维生素B2的浓度在1.0×10-6～8.0×10-4mol·L-1之间与其对应的氧化峰电流呈线性关系,检出限(3S/N)为2.5×10-8mol·L-1.方法用于维生素B2片的分析,测定值的相对标准偏差(n=5)在1.8％～2.7％之间,加标回收率在99.5％～102％之间.%Alizarin red S (ARS) was polymerized on the surface of carbon paste electrode (CPE) by CV,to prepare poly-ARS film modified CPE (ARS/CPE).Electrochemical behavior of vitamin B2 at this modified electrode was studied by CV and DPV.It was found that in a buffer medium of 0.1 mol · L-1 HOAc-NaAc solution of pH 4.6,significant electrocatalytic action toward the redox reaction of vitamin B2 was observed at the modified electrode,and values of diffusion coefficient (D) of 3.26 × 10 5 cm2 · s-1 and electron transfer coefficient (a) of 0.817 4 were found.Linear relationship between values of oxidation peak current and concentration of vitamin B2 was kept in the range of 1.0×10-6-8.0× 10-4mol · L-1,with detection limits (3S/N) of 2.5×10-8mol · L-1.The proposed method was applied to the analysis of vitamin B2 tablets,giving values of RSD's (n=5) in the range of 1.8％-2.7％.Recovery was tested by standard addition method,values of recovery found were ranged between 99.5％ and 102％.