目的 建立非水毛细管电泳法同时测定胃肠宁片中巴马汀、小檗碱和药根碱的含量.方法 以75 mmol/L醋酸铵甲醇溶液(pH 6.5)为电泳介质,运行电压15 kV,压力进样5s,柱温25℃,检测波长260 nm.结果 该方法能使3种药物在16 min内得到完全分离,巴马汀、小檗碱和药根碱分别在1.494～19.92 mg/L(r=0.9994)、1.485～19.8 mg/L(r=.0.9990)、和1.53～20.35 mg/L(r =0.9995)范围内线性关系良好；平均回收率分别为99.4％、100.5％、101.4％.RSD分别为0.40％、0.60％、0.30％.结论 本方法简单、可靠,灵敏度高,适用于胃肠宁片中巴马汀、小檗碱和药根碱的含量测定.%Objective To establish a non-aqueous capillary electrophoresis method for the separation and determination of palmatine ,berberine and jatrorrhizine in Weichangning Tablets. Methods The runing buffer was 75 mmol/L ammonium acetate (pH 6.5) ,the running voltage was 15 kV. The detection temperature was 25℃, and the detection wavelength was set at 260 nm. Results Linearity was obtained over the range of 1.494-19.92 mg/L( r = 0.9994)for palmatine, 1.485-19. 8 mg/L ( r =0.9990) forberberine and 1.53-20.35 mg/L( r =0.9995) forju-trorrhizine. The average recovery was 99.4% (RSD =0.4% ) for palmatine, 100.5% (RSD =0.6% ) for berber-ine and 101.4% (RSD =0.3% ) for jatrorrhizine, respectively. Conclusion This method is simple, rapid and reproducible. It is successfully employed for the determination of palmatine, berberine and jatrorrhizine in Weichangning Tablets.
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