采用液质联用法测定血浆中阿德福韦药物浓度.并优化阿德福韦耐药基因检测方法.药物浓度测定:0.2mL血浆中加入两倍体积的蛋白沉淀剂甲醇,高速离心后上清液吹干并用流动相甲醇-水(20:80)溶解,应用电喷雾离子源的质谱检测;用套式PCR快速检测病人阿德福韦耐药突变.两种方法适用于临床样本检测,并可结合用于临床个体化治疗的研究.%To develop a rapid and easy tO operating mechod for adefovir in plasma, and to optimize che method on identification of resistance genes, then to apply both methods on patients. Determination of drug concentration: The plasma samples were precipitated by methanol and centrifugated, the supernatants were volalilized by nilrogen and dissolved by mobile phase of methanol-water (20 : 80). Electrospray ionization source was applied and operated in the positive ion mode. Adefovir resisitance mutations in patients were delecled by nested PCR rapidly. The proposed method showed good linearily, repealabilily and recovery, and with the method on drug resistance genes it can be applied to the study of individualized therapy.
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