采用生理生化、激光拉曼和电镜扫描等方法进行添加不同浓度β-胡萝卜素对三角帆蚌早期珍珠色泽形成的研究。实验共分五组，I至V组β-胡萝卜素终浓度分别为0、0．000625％、0．00125％、0．0025％和0．005％。结果显示，当β-胡萝卜素的添加浓度为0．000625％时，SOD、POD的比活力均下降，而CAT的比活力升高；其早期珍珠在708cm、1088cm^-1处的CaCO3拉曼峰和1136cm-1、1528cm-1处的β-胡萝卜素拉曼峰强度值最高，分别为1761、6003和2630、3359，扫描电镜结果也显示此时珍珠层的文石结晶最好。结果表明，当三角帆蚌的生长水域中添加0．000625％浓度的β-胡萝卜素时，能够有效促进CaCO3文石规则有序地结晶，较好地提高三角帆蚌早期珍珠的色泽度。%Physiological and biochemical, laser Raman and scanning electron microscopy methods were used to investigate the effect of different concentrations of β-carotene on early pearl color formation in Hyriopsis cumingii. The experiment was divide into five groups, the last concentrations of β-carotene was 0, 0.000625%, 0.00125%, 0.0025% and 0.005% respectively. The results showed that SOD and POD enzyme specific activity were decreased, but CAT enzyme specific activity was increased when the concentration of β-carotene was 0.000625%. At the same concentration, the intensity values of CaCO3 laser Raman peak in 708cm-1 and 1088cm-1, β-carotene laser Raman peak in 1136cm-1 and 1528cm-1 were all the highest. They were 1761, 6003, 2630, 3359 respectively. The nacre aragonite crystal was also verified the best by scanning electron microscopy. The results suggested that the concentration of 0.000625% β-carotene can effectively promote the orderly crystallization of CaCO3 aragonite and optimum enhanced the early pearl＇s color and lustre derees in H. cumingii.
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