珠子参抗肝损伤药效的物质基础研究

摘要

Objective To study the therapeutic material basis of Panax japonicus in anti-hepatic injury .Methods Chemical composi-tions of Panax japonicus were extracted and separated by macroporous resin column chromatography .The active ingredients were screened by using the mice model of acute liver injury .The mice were divided into nine groups ,normal control ,model group ,posi-tive control ,the total saponins and polysaccharide of Panax japonicus group with high ,medium and low-dose .The activities of ALT ,AST were examined after the administration of drugs orally for 7 days .Results Compared with the model control group ,the total saponins with high and medium dose and polysaccharide of Panax j aponicus group with low-dose could significantly decrease the level of ALT ,AST ;chikusetsusaponin Ⅳa all-dose group and ginsenoside Ro with medium and low dose group could signifi-cantly decrease the concentration of ALT ,AST .Conclusion The total saponins and chikusetsusaponin Ⅳa were effective parts and active ingredients for anti-hepatic injury .%目的：探索珠子参抗肝损伤药效的物质基础。方法利用溶剂提取法、大孔树脂法及各种色谱技术，进行珠子参有效部位和化学成分的提取分离；采用CCl4所致小鼠化学性肝损伤模型，进行珠子参药效部位及有效成分筛选。将小鼠平均分为对照组、模型组、阳性对照组、珠子参不同剂量组，共9组。每天灌胃1次，连续7 d。检测血清中AST 和ALT含量的变化。结果珠子参总皂苷高剂量、中剂量可以降低ALT和AST含量，珠子参多糖低剂量组与模型组比较也可以降低ALT和AST 的含量，而总皂苷高剂量有显著作用；竹节参皂苷Ⅳa各剂量组、人参皂苷Ro中、低剂量组均能降低 ALT 和 AST 的含量，与模型组对比，差异有统计学意义，竹节参皂苷Ⅳa低剂量效果显著。结论珠子参总皂苷为珠子参抗肝损伤的有效部位，主要化学成分竹节参皂苷Ⅳa为其抗肝损伤的主要有效成分。