Objective To establish a fast method for simultaneous determination of geniposide and crocin‐1 in three kinds of tradi‐tional Chinese medicines by UPLC .Methods UPLC was adopted in this study .The analytical column was BEH C18 (50 mm × 2 .1 mm ,1 .7 μm) and gradient elution with methanol‐H2 O as mobile phase .The flow rate was 0 .3 mL · min-1 .The detection wavelength was set at 238 nm (0‐7 min) for geniposide and 440 nm (7‐20 min) for crocin‐1 .The column temperature was 30 ℃and the injection volume was 2 μL .Results The two components had a good linear relationship between 0 .010‐1 .000 mg · mL -1 and 0 .1‐10 .0 μg · mL -1 .The average recoveries (n=6) of geniposide and crocin‐1 in three preparations were 98 .2% ,99 .9% and 100 .3% (RSD 1 .3% ,1 .4% and 1 .0% ) and 99 .6% ,97 .4% and 98 .3% (RSD 1 .3% ,1 .4% and 1 .5% ) ,respectively .Conclu‐sion The separation of geniposide and crocin‐1 only needs 20 min with accuracy and good reliability .The method is simple ,specific and suitable for the simultaneous determination of the content of geniposide and crocin‐1 in the three preparations .%目的：建立快速测定栀子复方制剂复方牛黄消炎胶囊、清胃黄连丸和龙泽熊胆胶囊中栀子苷和西红花苷‐1的方法。方法采用U PLC法。色谱柱为BEH C18柱（50 mm ×2．1 mm ，1．7μm）；流动相：甲醇‐水以不同梯度洗脱；流速：0．3 mL · min－1；检测波长：0～7min为238nm，7～20min为440nm；柱温：30℃；进样量为2μL。结果3种复方制剂中栀子苷在0．010～1．000mg·mL－1范围内呈良好的线性关系（ r＝0．9999），平均回收率分别为98．2％，99．9％和100．3％，RSD分别为1．3％，1．4％和1．0％；西红花苷‐1在0．1～10．0μg · mL －1范围内呈良好的线性关系（r＝0．9999）。平均回收率分别为99．6％，97．4％和98．3％，RSD分别为1．3％，1．4％和1．5％。结论栀子苷和西红花苷‐1在20 min内获得良好分离，该方法简便、准确、快速，可用于栀子复方制剂中栀子苷和西红花苷‐1的测定。
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