目的 建立血浆中丹酚酸B、芍药苷和甘草酸的HPLC-MS检测方法,研究3种成分复配在大鼠体内的药动学参数特征.方法 尾静脉给予大鼠3种成分复配混合液,血浆样品采用液液萃取法处理,并以氯霉素为内标、用 HPLC-MS法测定血浆中3种成分的质量浓度.血药质量浓度数据用WinNonlin 7.0软件按照非房室模型处理,计算药动学参数,并采用SPSS 18.0统计软件进行分析.结果 建立了2种离子模式下同时检测3种成分的 HPLC-MS方法,丹酚酸B、芍药苷和甘草酸分别在0.02~6.25,0.01~4.38和0.04~12.50 mg·L -1内线性关系良好,方法回收率均在85% ~115% 之间,日内、日间精密度良好,稳定性符合要求.丹酚酸B和甘草酸的血药质量浓度-时间曲线分别显示1和2个吸收峰.大鼠体内丹酚酸B、芍药苷和甘草酸的 t1/2分别为23.11 ± 6.57,2.85 ± 0.83和6.71 ± 1.63 min,AUC0 ~ t分别为0.86 ± 0.05,9.84 ± 0.46和27.31 ± 1.68 mg·L -1·h.结论 建立的方法简便、快速,可用于3种成分联合用药的含量测定及药动学研究.%Objective To establish a method of high performance liquid chromatography-mass spectrometry(HPLC-MS)for the de-termination of salvianolic acid B(Sal B),paeoniflorin(PF)and glycyrrhizin(GL)in rat plasma,so as to discuss the pharmacoki-netic characteristics of the 3 compounds mixed administration in normal rats.Methods The rats were given the mixed compounds by caudal vein injection,and the blood samples were purified by liquid-liquid extraction with formic acid and acetonitrile.With chloramphenicol as an internal standard,HPLC-MS was adopted to determine the concentration of Sal B,PF and GL.The pharma-cokinetic parameters of the 3 compounds were calculated with WinNonlin 7.0 software and analyzed by SPSS 18.0 statistical soft-ware.Results The method of HPLC-MS analysis,which was used to measure the 3 components in 2 ion modes,was established. The linear ranges of Sal B,PF and GL were 0.02-6.25,0.01-4.38 and 0.04-12.50 mg·L -1,respectively.Good precision and sta-bility were showed and the average recoveries were all between 85%-115%.The plasma concentration time curves were drawn.It showed an absorption peak of Sal B and two absorption peaks of GL.The pharmacokinetic parameters of the 3 compounds were as follows:t1/223.11 ± 6.57,2.85 ± 0.83 and 6.71 ± 1.63 min,AUC0-twere 0.86 ± 0.05,9.84 ± 0.46 and 27.31 ± 1.68 mg·L -1·h. Conclusion The established method is simple and rapid,and can be used for the determination of the content of the combination of 3 components and the study of pharmacokinetics.
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