野桑蚕丝胶1基因Ser1A’及其上游调控序列的克隆和序列分析

摘要

家蚕由野桑蚕驯化而来，而野桑蚕sericin1及其上游调控序列的相关研究较少。本研究以家蚕sericin1及其上游调控序列设计引物克隆野桑蚕sericin1及其上游调控序列，将克隆得到的野桑蚕Sericin1序列翻译后用muscle软件与家蚕Sericin1蛋白序列比对，结果表明预测的野桑蚕Sericin1氨基酸序列与家蚕Sericin1A’（Ser1A’）氨基酸序列相似性很高，达98％。野桑蚕丝胶1基因上游调控序列与家蚕一样也存在多态性，克隆得到了106Ibp和636bp的两条序列，中间存在425bp的插入序列，与家蚕丝胶1基因上游调控序列相似性分别为98％和97％。%Bombyx mori was domesticated from B. mandarina. But there have been few researches of B. mandarina, and its sericinl and the upstream control sequence remain poorly understood. In the present study, we cloned the sericinl and the upstream control sequence of B. mandarina with the primers designed according to the sericinl and the upstream control sequences of B. mori. The amino acid sequence translated from the cloned sericinl of B. mandarina was aligned with Sericinl A＇ （Serl A＇） of B. mori, using the Muscle software. The result showed that the two sequences shared great similarity （about 98%）, which revealed that the cloned sericinl of B. mandarina can be designated as B. mandarina Sericin 1 A＇ （B. mandarina SerlA＇）. Like the case of B. mori, the upstream control sequence of B. mandarina also showed polymorphism. Two sequences, 1061 bp and 636 bp in length, with an insertion sequence 425 bp long, were cloned. They shared a similarity of 98% and 97% with sericinl and the upstream control sequence of B. rnori, respectively.