胰岛素样生长因子-1参与诱导小鼠诱导多潜能干细胞的研究

摘要

Objective:To study the effect of insulin-like growth factor-1 (IGF-1) on the induced efficiency of murine induced pluripotent stem(iPS) cells. Methods:Mouse embryonic fibroblasts were divided into control group and experimental group. The 2 groups were infected with retro-viruses expressing classic 4 factors while the medium of the experimental group was added IGF-1. Then the clones were determined. Result: The in-duced efficiency of the control group and the experimental group were (0.05± 0.0002)%and (0.01± 0.0005)%respectively. The clones were positive. Conclusion:IGF-1 improve the inducing efficiency of iPS cells.%　　目的：观察胰岛素样生长因子-1（IGF-1）对小鼠诱导多潜能干（iPS）细胞诱导效率的影响。方法：将鼠胚成纤维细胞分为对照组和实验组，2组均予经典四因子的逆转录病毒载体感染，实验组在培养基里加入IGF-1，进行iPS细胞诱导，并对其进行鉴定。结果：对照组和实验组的iPS细胞诱导效率分别为（0.05±0.0002）%和（0.01±0.0005）%，所获iPS细胞为阳性。结论：IGF-1提高小鼠iPS细胞的诱导效率。