微波辅助提取荔枝核黄酮类化合物及其抗氧化性研究

摘要

Microwave-assisted extract (MAE) process for flavonoids from litchi seeds was studied.Influences of solvent,microwave power,dosage of solvent,extracting time and extracting times on the extraction were investigated.From orthogonal experiments the optimum extracting parameters were determined as follows:solvents,60% alcohol;microwave power,700 W;irradiation time,150 s;material:solvents,1∶ 25 (g/mL);extracting times,one.Under these conditions the yield of flavonoids was 6.86% and the content of flavonoids in extracts reached 36.7%.Compared with the conventional extraction methods,the MAE process provided high extraction rate,high extraction selectivity and required short time.Antioxidant experiments showed that the flavonoids from litchi seeds had strong hydroxyl free radical (OH·) and superoxide anion free radical (O2-·) scavenging activities.%研究微波辅助法提取荔枝核黄酮类化合物的工艺,考察了提取溶剂、微波功率、溶剂用量、辐射时间、提取次数等因素对提取的影响.通过正交实验确定最佳的提取参数为:60%乙醇作为提取溶剂,微波功率700 W,料液比1∶ 25,辐射时间150 s ,提取一次.在此优化条件下用微波辅助,黄酮类化合物的得率为6.86%,提取物中黄酮含量达到36.7%.抗氧化性研究表明荔枝核黄酮类化合物有良好的抗氧化活性,能有效清除羟基自由基(OH·)和超氧阴离子自由基(O2-·).