本文用两步萃取法从菜籽粕脱毒液中提取硫代葡萄糖苷,以3,5-二硝基水杨酸比色法测定硫代葡萄糖苷含量,采用单因素试验法,分别考察可能影响硫代葡萄糖苷提取效果的各种因素,确定了最佳工艺条件:第一步萃取剂为异辛烷,母液与异辛烷体积比为2:1,搅拌时间为30 min,异辛烷萃取次数为2次,异辛烷单次萃取率达71.2%.硫代葡萄糖苷收率为83.7%.%This paper discussed the extraction of glucosinolates from rapeseed meal detoxifying solution. The content of glucosinolates was determined by 3,5-dinitrosalicylic acid colorimiay and the various factors responsible for the effect of extraction of glucosinolates were investigated respectively by single factor method. The most suitable extraction conditions were as follows:in the first step,where the volume ratio of mother solution to extractant, which was isooctane,was 2: 1, extraction yield reached 71.2% in the first extraction by isooctane. In this step,there were two extractions, each of which lasted for 30 min. The total recovery of glucosinolates was 83.7%.
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