桦木酮酸对肿瘤细胞SGC-7901、HepG-2及S_(180)荷瘤小鼠肿瘤细胞的影响

摘要

To study the effects of betulonic acid on SGC-7901 ,HepG-2 in vitro and mice of bearing S_(180) tumor cells in vivo ,the effects of betulonic acid on tumor cell of SGC-7901 and HepG-2 were observed by MTT and colony formation. The morphocytology of S_(180) tumor cells was observed by HE stain. It has been found that betulonic acid can inhibit the growth of tumor cell SGC-7901 and HepG-2 in vitro studies. Their IC_(50) were 68.14 and 110.77 μmol/L respectively. The inhibition rate on S_(180) tumor cells were 68. 3% and 41.5% , respectively after being administered orally for 8 consecutive days at 150 and 75 mg/kg/d (P < 0.05 ). The morphocytology of S_(180) tumor cells had exhibited an obvious oncosis, such as cell light staining,increased cell volume and the intact nucleus structure. Betulonic acid has definite action on tumor cells and the mechanism may be induced cell oncosis.%为研究桦木酮酸体外对SCC-7901、HepG-2及体内对S_(180)荷瘤小鼠的影响,采用MTT与肿瘤细胞集落形成能力实验观察桦木酮酸对HepC-2和SGC-7901作用.通过抑瘤率观察其对S_(180)荷瘤小鼠的影响;HE染色观察其对S_(180)肿瘤细胞形态学的影响.结果显示:桦木酮酸可抑HepG-2和SCC-7901细胞的生长,MTT与肿瘤集落形成能力实验测得其IC_(50)分别为68.14和110.77 μmol/L.连续给药8 d后,150和75 mg/kg/d剂量的桦木酮酸对S_(180)的抑制率分别为68.3%和41.5%(P<0.05);HE染色发现肿瘤细胞表现较为明显的胀亡现象:细胞淡染、细胞体积增大,细胞核结构相对完整.提示桦木酮酸体内、外对肿瘤细胞均有一定的抑制作用,其作用可能与其引起肿瘤细胞胀亡有关.