采用单因素实验法,以树脂的饱和吸附量、产品的得率及纯度为指标,考察各因素对动态吸附与解吸的影响,优化大孔树脂动态吸附分离无患子皂苷的工艺条件.结果表明,当pH为4.5的无患子皂苷水提液以流速1 mL/min通过高径比为5.4∶1的吸附柱时,AB-8树脂对无患子皂苷的饱和吸附量达568 mg/g；采用1.5 BV的95％乙醇以1 mL/min的流速洗脱吸附后的树脂,产品得率为83.48％、纯度达93.00％；树脂重复使用8次后其吸附解吸性能基本不变.该方法可以用于无患子皂苷的工业化分离提纯.%To optimize the dynamic adsorption and desorption process parameters for sapindus-saponins onto AB-8 resin, the monofactorial experiments were used to analyze the effects of the process parameters on the saturated adsorption capacity, the recovery yield and the purity of the sapindus-saponins. The results showed that the saturation adsorption capacity of sapindus-saponins on AB-8 resin achieved 568 mg/g when the crude sapindus- saponins water extracts ( pH4. 5 ) flowed through the resin column (the ratio of height to diameter 5.4:1) at 1 mL/min. The elution parameters were as follows; 1-5 BV 95% ethanol aqueous solutions at 1 mL/min. The sapindus-saponins content in the final product was 93% with a recovery yield of 83.48%. In addition,the stability of AB-8 resin was fine after eight-time recycled. This method could provide a potential approach for large-scale separation and purification of sapindus-saponins for its wide pharmaceutical use.
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