中国蛇岛蝮蛇毒腺cDNA文库ESTs序列测定及生物信息学分析

摘要

前期我们构建了中国蛇岛蝮蛇(Gloydius shedaoensis shedaoensis,GSS)毒腺(GSSG)的cDNA(GSSG-cDNA)文库.本文从构建的GSSG-cDNA文库阳性重组子中随机挑选了216个单克隆进行5'端表达序列标签(EST)单向测序,获得了211条高质量的ESTs.生物信息学序列比对分析结果表明84个克隆为已知功能基因,29个克隆为未知功能基因,98个克隆为新基因,分别占总ESTs的39.8％、13.7％和46.5％.成功获得了GSSG的部分ESTs序列,为GSS蛋白活性组分基因的克隆、表达和功能研究奠定了一定基础.%Previously, we have successfully constructed a cDNA library of Chinese Gloydius shedaoensis shedaoensis (GSS) venom gland (GSSG). In current work,a total of 216 GSSG-cDNAs were randomly picked up and analyzed by single-pass sequencing from the 5' end. A total of 211 ESTs in high quality were generated and sequenced. Bioinformatics sequencing blasting results indicated that 84 ESTs could be annotated as the genes with known function,29 ESTs as similar genes with unknown function,and the rest of 98 ESTs were identified as novel genes,which account 39. 8% ,13.7% and 46. 5% of 211 obtained ESTs,respectively. Taken together,the partial ESTs of GSSG were obtained in current work, which provides certain useful information for cloning and expressing target protein genes and studying the biological functions of target proteins from GSS.