银耳多糖单糖组成分析的三种色谱方法比较

摘要

To find an accurate and fast method to determine the monosaccharide composition of Tremella polysaccharide, thin layer chromatography ( TLC ) , gas chromatography ( GC) and high performance liquid chromatography ( HPLC ) were compared. The results of TLC and GC were not ideal. However,HPLC was a simple method with high sensitivity, good resolution and integrity information. The result of HPLC analysis showed that the monosaccharide compositions were Glc,Man,GlcA,Xyl and Fuc.with the mole percentage of 0. 24:1.00= 0.06:0. 29: 0. 25. Consequently, HPLC is the most suitable method for moncsaccharide composition analysis.%为选择一种准确快捷的方法测定银耳多糖的单糖组成,对薄层色谱法(TLC)、气相色谱法(GC)、高效液相色谱法(HPLC)三种色谱方法进行比较.结果表明,前两种方法的测定结果均不理想,而HPLC法,操作简便,灵敏度高,分离效果好,信息完整.测定结果为由葡萄糖、甘露糖、葡萄糖醛酸、木糖、岩藻糖组成,其摩尔比为0.24∶1.00∶0.06∶0.29∶0.25.HPLC法对酸性杂多糖组成糖分析是一种比较理想的选择.