Monosaccharide compositional analysis of purified polysaccharide from Tricholoma matsutake by capillary gas chromatography

摘要

Two polysaccharide (MTS-1 and MTS-2) were isolated from the water extract of?Tricholoma matsutake?and purified through ethanol precipitation, deproteination, DEAE-52 cellulose anion exchange and Sephadex G-100 gel-filtration chromatography. Their molecular weight was determined using high performance liquid chromatography and gel permeation chromatography (HPGPC) and their monosaccharide composition was analyzed by GC methods. It was shown that MTS-1 was composed of glucose, xylose, galactose in a molar ratio of 12.89:1.20:1 and MTS-2 consisted of glucose unit only. The molecular weights (Mw) of MTS-1 and MTS-2 were 5,939a and 232,317 Da, respectively. In the GC method for the analysis of monosaccharide composition of purified polysaccharide in?Tricholoma matsutake, the peak area of each monosaccharide had a good linear relationship with its concentration (r2>0.99). The intermediary precision values (expressed as the RSD) were less than 2%.The average recoveries of glucose, galactose and xylose ranged from 99.86 to 104.26% with RSD values of less than 5%. This developed method was accurate and had good reproducibility and could be used to determine the monosaccharide contents of?T. matsutake.