蜂毒肽对成熟树突细胞诱导T细胞分化的免疫调节的影响

摘要

To study the intervention of melittin (MT) on dendritic cells (DC) co-cultured with lymphocytes stimulated by lipopolysaccharide (LPS),the impact of MT on the differentiation of Th1/Th2 was investigated.MTS was used to detect the activity of DC co-cultured with lymphocytes by LPS and MT;Flow cytometry was used to detect the ratio of Th1 and Th2 in the co-culture of DCs stimulated by MT and LPS;ELISA was used to detect the concentration IL-4,INF-γin cell supernatant of MT and LPS stimulated co-cultured DC and lymphocyte.The results showed that a certain concentration of MT and LPS can stimulate the co-cultured activity of DC with lymphocytes.In the induction of mature DC,MT can increase the Th1/Th2 ratio,the Th1 percentage (P ＜ 0.05) and the IFN-γ (P ＜ 0.01).But it had no effect on the concentration of IL-4 and Th2 (P ＞ 0.05).The study demonstrated that through upregulating the expression of Th1 cell ratio and IFN-γconcentration,MT promoted the differentiation of Th1/Th2 cells to the Th1 pathway,thus playing immunomodulatory effects on T cells.%通过蜂毒肽(Melittin,MT)干预脂多糖(Lipopolysaccharide,LPS)刺激树突细胞(Dendritic Cell,DC)与淋巴细胞共培养,以探究MT对Th1/Th2分化的影响.用MTS检测LPS、MT对DC与淋巴细胞共培养活性;流式细胞术检测MT对LPS刺激DC与淋巴细胞共培养中Th1、Th2比例;ELISA检测LPS刺激DC与淋巴细胞共培养中细胞上清液中IL-4、INF-γ浓度.结果显示MT、LPS在一定浓度下刺激DC与淋巴细胞共培养的活性;在成熟的DC诱导下,MT能上调Th1/Th2比例、Th1百分率(P＜0.05)及IFN-γ浓度(P＜0.01);对IL-4浓度、Th2百分率无影响(P＞0.05).本研究说明MT通过上调Th1细胞比例,IFN-γ浓度,促进Th1/Th2细胞向Th1方向分化途径对T细胞起免疫调节作用.