胰岛素样生长因子1对大鼠肾间质纤维化组织中CTGF的表达影响

摘要

目的观察胰岛素样生长因子1(IGF-1)对肾间质纤维化大鼠结缔组织生长因子(CTGF)表达的影响。方法30只Wistar大鼠随机分为假手术组、模型组、IGF-1组，每组10只。应用单侧输尿管结扎制备肾间质纤大鼠模型。假手术组打开腹腔后即缝合；模型组每日腹腔注射生理盐水0.5ml；IGF-1组腹腔注射重组人IGF-1750ng·kg-1·d-1。术后第14d麻醉大鼠后取材，检测肾功能，观察Ⅳ型胶原蛋白、结缔组织生长因子(CTGF)的表达。结果模型组血肌酐、尿素氮、Ⅳ型胶原蛋白、CTGF的表达水平均高于假手术组，差异有统计学意义(P <0.05)；IGF-1干预后，IGF-1组血尿素氮、血肌酐、Ⅳ型胶原蛋白、CTGF的表达水平均高于假手术组及模型组，差异有统计学意义(P <0.05)。结论 IGF-1参与梗阻性肾病的病理生理过程，尤其在肾间质纤维增生过程中起着重要作用。%　　Objective To investigate the effects and mechanisms of IGF-1 in rats with Unilatreal Ureteral Obstruction. Methods UUO rat model treated with IGF-1as an active treatment with recognized renoprotective BUN, Ser were measured The expression of col-Ⅳ, CTGF a in kidneys were observed. Result The expression of BUN, Scr, col-Ⅳ, CTGF the renal interstitial fibrotic area were highter significantly than that in sham group. Compared with UUO group, those of the IGF-1groups increased markedly(P <0.05). The level of BUN, Scr, col-Ⅳ, CTGF in UUO group were significantly highter than that in sham and uuo group(P <0.05). Conclusion IGF-1 in the pathophysiology of obstructive nephropathy in the process, especially in the renal interstitial fibrosis plays an important role.