The preparation of a novel silica adsorbent with nanopores and its selective adsorption for genistein from crude solution are investigated. According to the molecular recognizing technique, the silica adsorbent was prepared using genistein as the template molecule, tetraethyl orthosilicate as the cross-linker and γ-aminopropyltriethoxysilane as the functional monomer which reacted to the phenol hydroxyl group on genistein. It was evaluated by the Brunauer-Emmett-Teller (BET), Fourier transform infrared spectroscopy (FF-IR), transmission electron microscope (TEM) measurements, the static adsorption experiment, selective adsorption experiment and elution experiment. The results show that the silica adsorbent has high specific area, special selectivity for genistein, and the recognition performance for template molecular in the polar solution. There are lots of nanopores, which match the shape of the template molecular, uniformly distributed in the surface of silica adsorbent. New bonds are formed between genistein and functional monomer. It is concluded that the ability of the silica adsorbent to bind genistein is a result of both specific size and shape of the nanopores consisting with the correct position of the functional groups involved in the template binding through ionic and hydrogen interactions, and the nanopores lead to the best performance in the adsorption experiment.%为了将染料木素从其粗溶液中精确分离出来,制备了一种纳米结构硅质吸附剂.它根据分子识别技术,以染料木素为模板剂,氨丙基三乙氧基硅烷为功能单体,正硅酸乙酯为偶联剂,其中功能单体可以与染料木素的酚羟基发生反应.吸附剂经过BET法、红外分析(FT-IR)和透射电镜(TEM)表征,以及静态吸附实验、选择性实验和洗脱实验.结果表明,此吸附剂有高的比表面积,对染料木素有专一的选择性,能在极性溶剂中识别模板分子;有大量的与模板分子相匹配的纳米孔分布在吸附剂表面,并且有新的化学键形成.由此可以看出,通过模板分子与功能单体强烈的电荷和氢键作用,吸附剂能够专一键合染料木素,而纳米孔则大大提高了吸附能力.
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