人成骨样MG63细胞中CTGF降表达对基质金属蛋白酶－1表达的影响

摘要

objective to observe the osteogenetic man MG63 cells after CTGF expression reduction of matrix metalloproteinase 1 (MMP 1) expression. Methods for people CTGFmRNA design and synthesis of three siRNA in 21 nucleotides (siRNA1, siRNA2, siRNA3);Using cationic liposome (DMRIE- C reagent) respectively to three CTGFsiRNA osteogenesis MG63 cell transfection into people, with a blank and nonspecific siRNA in comparison, collecting cells after transfection 48 h. Results compared with the blank control group, transfection siRNA1, siRNA3 CTGFmRNA MG63 cells and protein decreased significantly, transfection siRNA2 CTGF expression and nonspecific siRNA MG63 cells have no obvious change. Transfection siRNA1 siRNA3 MG63 cells MMP 1 mrna and protein expression significantly lowered. Conclusion CTGFmRNA design and synthesis of siRNA against people can effectively restrain the person osteogenesis MG63 cell the transcription and expression of CTGF.%观察人成骨样MG63细胞中CTGF降表达后对基质金属蛋白酶－1（MMP－1）表达的影响。针对人CTGFmRNA设计、合成三对21核苷酸siRNA（siRNA1、siRNA2、siRNA3）；使用阳离子脂质体（DMRIE－C reagent）分别将三对CTGFsiRNA转染入人成骨样MG63细胞，以空白及非特异性siRNA作为对照，转染48h后收集细胞。与空白对照组相比，转染siRNA1、siRNA3的MG63细胞CTGFmRNA和蛋白显著降低，转染siRNA2及非特异性siRNA的MG63细胞CTGF的表达无明显变化。转染siRNA1、siRNA3的MG63细胞MMP－1mRNA和蛋白表达明显下调。针对人CTGFmRNA设计、合成的siRNA可有效抑制人成骨样MG63细胞CTGF的转录和表达。