辛伐他汀诱导人胃癌SGC7901细胞凋亡及其对Bax和Bcl-2表达的影响

摘要

目的 研究辛伐他汀对人胃低分化腺癌细胞株SGC7901凋亡的影响及其可能的分子机制.方法 体外培养人胃癌SGC7901细胞至对数生长期, 再分别用不同浓度的辛伐他汀处理SGC7901细胞,48h后采用流式细胞术检测细胞凋亡;RT-PCR法和Western Blot法观察Bax和Bcl-2的表达.结果 辛伐他汀能诱导SGC7901细胞凋亡,且呈浓度依赖性.流式细胞术检测显示10、20、40μmol/L辛伐他汀组细胞凋亡率分别为(20.37±3.60)%、(35.17±3.91)%、(58.39±4.06)%,与对照组(4.78±1.51)%相比,凋亡率显著增强(P＜0.05).不同浓度的辛伐他汀作用后能显著增强SGC7901细胞Bax mRNA和蛋白的表达,降低Bcl-2 mRNA和蛋白的表达(P＜0.05). 结论 辛伐他汀呈浓度依赖性地诱导胃癌SGC7901细胞凋亡,其机制可能与上调Bax、下调Bcl-2表达有关.%Objective To investigate the effects of Simvastatin on apoptosis in human gastric lower-differentiation ade-nocarcinoma cell line SGC7901 and to explore its potential molecular mechanisms. Methods Gastric SGC7901 cells were cultured in vitro, cells of exponential phase of growth were used to experiment. Then using different concentrations of Simvas-tatin role in SGC7901, 48 hours later, flow cytometry method was used to detect the cell apoptosis, the expressions of Bax and Bcl-2 were tested by RT-PCR and Western-Blot assay. Results Simvastatin could promote the apoptosis of gastric cell line SGC7901 in a dose-dependent manner. Flow cytometry method showed that,Simvastatin (10μmol/L, 20μmol/L, 40μmol/L) role in SGC7901 for 48 hours, the apoptosis rates of the three groups were (20.37±3.60)%,(35.17±3.91)%,(58.39±4.06)%, the apoptosis rate of the control group was (4.78±1.51)%, the experimental groups were significantly higher than the control group (P＜0.05). Different concentrations of Simvastatin could promote the expression of mRNA and protein of Bax, reduce the expres-sion of mRNA and protein of Bcl-2 in SGC7901 cells(P＜0.05). Conclusion Simvastatin can promote the apoptosis of gastric cell line SGC7901 in a dose-dependent manner. One of the possible mechanisms of apoptosis is that Simvastatin can improve the expression of Bax and reduce the expression of Bcl-2.