三七总皂苷联合雷公藤多苷对关节炎大鼠外周血 TNF－α、IL－6、MCP－1以及胸主动脉 M CP －1表达的影响

摘要

目的：观察中药有效成分三七总皂苷联合雷公藤多苷对胶原诱导性关节炎（CIA）大鼠模型外周血肿瘤坏死因子－α（TNF－α）、白细胞介素－6（IL－6）、单核细胞趋化蛋白－1（ MCP－1）以及胸主动脉MCP－1表达的影响。方法从50只大鼠中随机选取8只作为正常组，其余大鼠建立CIA模型。将成模大鼠随机分为模型组、雷公藤多苷组、联合组、甲氨蝶呤组，每组8只。雷公藤多苷组给予雷公藤多苷10 mg／（ kg· d）灌胃，联合组给予三七总皂苷10 mg／（kg· d）和雷公藤多苷200 mg／（kg· d）灌胃，甲氨蝶呤组给予甲氨蝶呤1．6 mg／（ kg· d）灌胃，正常组和模型组给予等量生理盐水灌胃，均1次／d，共42 d。灌胃结束后次日应用液相蛋白芯片技术检测血清中TNF－α、IL－6、MCP－1水平，应用免疫组织化学法检测胸主动脉MCP－1的表达量。结果模型组血清中TNF－α、IL－6、MCP－1水平均明显高于正常组（P均＜0．05），各给药组均明显低于模型组（P均＜0．05），联合组均明显低于甲氨蝶呤组和雷公藤多苷组（P均＜0．05），甲氨蝶呤组和雷公藤多苷组比较差异无统计学意义（P均＞0．05）。模型组MCP－1表达量明显低于正常组（P＜0．05），各给药组MCP－1表达量均明显高于模型组（P均＜0．05），联合组明显高于甲氨蝶呤组和雷公藤多苷组（P均＜0．05），甲氨蝶呤组和雷公藤多苷组比较差异无统计学意义（P＞0．05）。结论三七总皂苷联合雷公藤多苷可明显降低血清炎症因子TNF－α、IL－6、MCP－1水平，上调MCP－1在胸主动脉的表达，且较单用雷公藤多苷或甲氨蝶呤效果更优，提示中药有效成分配伍在抗炎、保护心血管方面具有增效作用。%Objective It is to detect the active ingredients of traditional Chinese medicine PNS combined with Tripterygium glycosides (TG) on tumor necrosis factor (TNF-α), interleukin-6(IL-6),monocyte chemotactic protein -1(MCP-1) levels in serum of CIA rats .Methods 50 rats were selected in which 8 rats as normal group and the other rats were used to es-tablish CIA models, then the models were randomly divided into model group ,TG group, combined group, Methotrexate group (MTX), each group included 8 samples.Normal group and model group were given equivalent volume of saline .TG group was given TG 10 mg/(kg· d) by gavage, combined group given TG 200 mg/(kg· d) +PNS 10 mg/(kg· d) by gavage, MTX group given MTX 1.6mg(kg· d), all the groups were treated for 49 days.On 49nd day after modeling, protein chip technology was used to measure TNF -α, IL-6,MCP-1 levels in serum, the expression of MCP -1 in thoracic aorta were detected by immunohistochemical method .Results The levels of serum TNF-α,IL-6, MCP-1 in model group were higher than that in normal group (P all<0.05), the levels in every treated group were lower than that in model group (P all<0. 05), those in combined group were lower than that in TG group and MTX group (P all<0.05).No significant difference was found between TG group and MTX group (P>0.05).The expression of MCP -1 in thoracic aorta was lower in model group than that in normal group (P all<0.05), the levels in every treated group were higher than that in model group (P all<0. 05), those in combined group were higher than that in TG group and MTX group (P all<0.05).No significant difference was found between TG group and MTX group (P>0.05).Conclusion PNS+TG can significantly reduce the levels of TNF -α, IL-6,MCP-1 in serum,up-regulate the level of MCP-1 in thoracic aorta, and the effect was more significantly than sim-ple drug, which indicate that compatibility of traditional Chinese medicine active ingredients has a synergistic effect on anti -inflammatory and the aspect of prevention of cardiovascular damage .