骨髓间充质干细胞对糖尿病视网膜病变大鼠血糖及视网膜功能影响

摘要

目的：探讨尾静脉注射骨髓间充质干细胞对糖尿病视网膜病变大鼠血糖及视网膜功能的影响。方法：48只健康大鼠随机选取10只为正常对照组，38只建立糖尿病视网膜病变大鼠模型后，20只采用尾静脉注射间充质干细胞，18只注射等量安慰剂。观察间充质干细胞在肝、脾、肾、胰腺和眼球的分布以及在视网膜的分化特点，监测注射后1、2、3、4wk血糖变化，观察血-视网膜屏障破坏修复情况。结果：BMSCS在肝、脾、肾、胰腺、肺等组织中均有分布，视网膜外核层可见到大量密集的BMSCs重叠存在。实验组大鼠的血糖水平均随干预时间延长逐渐下降，注射后第1 wk血糖差异即有统计学意义。EB渗漏量较基线明显减少。结论：BMSCs可降低糖尿病视网膜病变大鼠血糖，使血-视网膜屏障得到一定程度修复。%Objective:To discussion the influence of blood glucose in diabetic retinopathy and retinal function in rats when injected the bone marrow mesenchymal stem cells on tail vein. Methods:Selecting 10 from the 48 rats randomly to be as normal control group, 38 rats after the establishment of diabetic retinopathy lesions, 20 using tail vein injection of mesenchymal stem cells and 18 the same amount of a placebo injection. Charge distribution was observed between mesenchymal stem cells in the liver, spleen, kidney, pancreas, and the characteristics of the eye and the retina differentiation. Observing the blood-retinal barrier breakdown repair the situation after1,2,3,4 weeks’ injection of blood glucose monitoring. Results:BMSCS are distributed in the liver, spleen, kidney, pancreas, and a large number of overlapping dense BMSCs can be seen in outer nuclear layer of the retina. Blood glucose average experimental rats gradually decreased with prolonged intervention, 1 week after injection blood sugar difference that is statistically signiifcant. EB leakage was signiifcantly reduced compared with baseline. Conclusion:BMSCs reduces blood glucose in diabetic rats with retinal lesions, and the blood-retinal barrier can be extent repaired.