大黄和栀子对脾胃虚寒实验大鼠模型的影响

摘要

Objective:To observe the effect of two common Chinese medicine Rheum officinale and Gardenia jasminoides,on spleen and stomach cold experimental model in rat.Methods:The spleen and stomach cold experimental model in rats was copied.Different doses of extracts of two drugs as the test material,weight index,the stomach gastric emptying rate, malondialdehyde (MDA ) were measured, and enzyme-linked immunoassay has been applied to the determination of serum gastric gastrin-releasing (GAS ),MTL and PGE2 in different groups.Results:All indicatiors in Rh.officinale high dose group showed no significant difference compared with model group.PGE2 in Rh.officinale medium dose group had significant difference compared with model group.Gastric emptying rate and GAS,in Rh.officinale low dose group had significant difference,compared with model group,the PGE2 and MTL had a very significant difference.All indexes in G.jasminoides high and low dose groups showed no significant difference compared with model group,all indexes in G.jasminoides medium dose group showed significant difference compared with model group,and the MDA has very significant difference.Conclusion:Rh.officinale low dose has good treatment effect on spleen deficiency rats,high and middle dose shows no therapeutic effect.G.jasminoides medium dose has good treatment effect on spleen deficiency rats,high and low dose show no therapeutic effect.%目的：观察常见两种苦寒中药大黄、栀子不同剂量提取液对脾胃虚寒实验大鼠模型的影响。方法：制备脾胃虚寒实验大鼠模型，以两种苦寒中药不同剂量的提取液为受试物，检测胃重指数、胃排空率、丙二醛（MDA）以及利用酶联免疫法测定血清当中的胃泌素（GAS）、胃动素（MTL）和前列腺素（PGE2）指标在不同组别的变化情况。结果：大黄高剂量组所有指标与模型组比较差异没有统计学意义，大黄中剂量组的 PGE2与模型组比较差异有统计学意义，大黄低剂量组的胃排空率和 GAS 与模型组比较差异有统计学意义，PGE2和 MTL 差异有统计学意义。栀子高剂量组的所有指标与模型组比较差异均无统计学意义，栀子中剂量组的所有指标与模型组比较差异均有统计学意义。栀子低剂量组的所有指标与模型组比较差异均无统计学意义。结论：大黄低剂量组对脾胃虚寒大鼠有很好的治疗作用，有健胃效应，而高、中剂量组无治疗作用。栀子的中剂量组对脾胃虚寒大鼠有很好的治疗作用，有健胃效应。栀子高、低剂量组没有治疗作用。