野生和家种蒲公英质量比较研究

摘要

Objective:To compare the quality of the wild and cultivated Taraxacum mongolicum.Methods:The content of chlorogenic acid and caffeic acid was determined by a rapid HPLC method.The content of total flavonoids and polysaccharide was determined by UV-visible spectrophotometry.Results and Conclusion:There was no significant difference in the contents of caffeic acid,chlorogenic acid and flavonoids between the wild and cultivated T.mongolicum,but the content of polysacharide in the wild was significantly higher than in the cultivated,for the content of polysaccharide specially in the root was higher than that in other parts.The coffee acid may not be suitable as a quality control factor.It is suggested to have the content of polysaccharide as a quality control index.%目的:比较家种和野生蒲公英的质量.方法:选择了4个产地的野生和家种蒲公英样品,高效液相色谱法测定其中咖啡酸和绿原酸含量,紫外-可见分光光度法测定其中多糖和总黄酮含量.结果与结论:咖啡酸、绿原酸和总黄酮在野生品和家种品之间没有显著差异,野生品的多糖含量显著高于家种品,因为野生品带根,根中多糖含量显著高于其他部位;咖啡酸作为蒲公英的质量控制成分可能不适合,建议增加蒲公英有效成分之一多糖的含量测定作为质量控制指标.