目的:探讨茯苓不同提取部位对小鼠胃肠运动功能的抑制作用,并筛选出最佳提取部位.方法:采用乙酰胆碱(acetyl choline;Ach)诱导的小鼠离体小肠痉挛性收缩模型,以小肠平均张力(mean contractile force;MCF)、收缩频率(contraction frequency;CF)为指标,在离体器官水平上比较茯苓水提液、茯苓25%醇提液、茯苓50%醇提液、茯苓75%醇提液、茯苓100%醇提液对离体小肠收缩的抑制作用;采用新斯的明诱导的胃肠功能亢进小鼠模型,以胃残留率和小肠推进率为指标,进一步验证最佳部位茯苓50%醇提液对小鼠胃排空和小肠推进的抑制作用.结果:茯苓水提液,茯苓25%、50%、75%和100%醇提液均有效缓解Ach诱导的离体小肠痉挛性收缩,显著降低小肠MCF而对CF影响较小,作用强度顺序为:茯苓50%醇提液>茯苓25%醇提液>茯苓75%醇提液>茯苓100%醇提液>茯苓水提液;茯苓50%醇提液低、中、高剂量均有效缓解新斯的明诱导的小鼠胃肠功能亢进,提高胃残留率,降低小肠推进率,并呈剂量依赖性关系.结论:茯苓水提液,茯苓25%、50%、75%、100%醇提液对小鼠离体小肠痉挛性收缩均有一定的抑制作用,其中50%醇提液作用最强,且对新斯的明引起的小鼠胃肠功能亢进有显著的拮抗作用.%Objective:To investigate the relaxing effects among the five extracts of Poriaon gastrointestinal motility in mice and to discover the optimal extractMethods:Tetanic contraction mice small intestine induced by acetyl choline (Ach) was adopted to evaluate the spasmolytic activities among the aqueous extract,25% alcohol extract,50% alcohol extract,75% alcohol extract and 100% alcohol extract of Poria in vitro,and the mean contractile force (MCF) and the contraction frequency (CF) were investigatedThen,the stomach residue rate and the small intestine propulsive rate were evaluated on gastrointestinal motility hyper-function mice induced by neostigmine after orally giving 50% alcohol extract of PoriaResults:All of the aqueous extract,25% alcohol extract,50% alcohol extract,75% alcohol extract and 100% alcohol extract of Poria reversed the small intestine tetanic contraction induced by Ach reducing the MCF with no inhibition on the CFBesides,all of the low,middle and high doses of the 50% alcohol extract of Poria improved the stomach residue rate and reduced the intestine propulsive rate with dose-dependent relationConclusion:All of the five extracts of Poria could significantly antagonized mice small intestine tetanic contraction induced by Ach in vitro,while the 50% alcohol extract of Poria was of the most efficient action,and the 50% alcohol extract of Poria reinstated the gastrointestinal motility of mice induced by neostigmine in vivo.
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