左旋龙脑对UVB辐射后小鼠皮肤光损伤的影响

摘要

Objective:To study effects of L-borneol on interleukin-6 (IL-6)and nuclear factor kappa-B (NF-κB)in UVB irradiation-induced mice.Methods:Eighty of Balb/c mice were randomly divided into four groups.Photo-damaged mouse model was established by UVB irradiation.L-borneol was adopted for wounds once every day for 11 days.The pathological changes of the photo-damaged skin tissue were observed by HE staining.Samples were collected at different time points after UVB irradiation to assess healing time,tissues water content,erythema and edema.The levels of 8-hydroxy-desoxyguanosine (8-OHdG),IL-6 and NF-κB in the epidermis were detected by ELISA.Results:Compared with the normal group,the erythema and edema indexes increased significantly in the UVB model group.The erythema and edema indexes,IL-6 and NF-κB levels in mice with acute UVB exposure significantly reduced after treatment with L-borneol.Further more,HE staining showed that UVB irradiation induced keratinocyte hyperplasia,sunburn cell formation and inflammatory cell infiltration.After treatment with L-borneol,these histological abnormalities were alleviated.In addition,the topical application of L-borneol resulted in a significant decrease in UVB-induced increases of epidermis thickness,especially depressing in UVB mediated generation of 8-OHdG(P ＜ 0.05,P ＜ 0.01).Conclusion:It is considered that L-borneol could alleviate the symptoms of photodamages by UVB irradiation.One principle of the effects could be that L-borneol removed of light products 8-OHdG,than controlled NF-κB regulation on release of IL-6 and lead to fall of IL-6 level in photo-damaged tissue causing role of anti-photo-damage.%目的:研究左旋龙脑对中波紫外线(UVB)照射后小鼠皮肤光损伤的影响.方法:通过UVB照射Balb/c小鼠建立光损伤模型,创面外用左旋龙脑,每天1次,连续给药11 d;在伤后不同时相点取材,HE染色观察皮肤组织的病理变化,对皮肤红斑、水肿反应进行评分;测定左旋龙脑对创面愈合时间、组织含水量、8-OHdG、IL-6和NF-κB的影响.结果:与正常组相比,光损伤小鼠红斑、水肿指数明显增加;与模型组相比,左旋龙脑组红斑、水肿指数明显减轻,结痂脱落时间减少,皮肤组织中8-OHdG、IL-6和NF-κB的水平显著降低(P ＜0.05,P＜0.01);HE染色显示模型小鼠皮肤表皮增厚明显,伴炎性细胞浸润,经左旋龙脑处理后,上述病理改变减轻.结论:左旋龙脑可能通过清除光产物8-OHdG、抑制NF-κB对IL-6的释放的调节作用,使光损伤组织中IL-6下降而起抗炎作用,促进光损伤伤口愈合.