葛根素在水溶液中的降解动力学研究

摘要

目的:研究葛根素水溶液在不同的pH、温度、离子强度、初始浓度条件下降解反应的动力学特征.方法:采用高效液相色谱法考察不同条件下葛根素含量随时间的变化,利用化学反应动力学的方法计算葛根素在不同环境下的降解反应动力学参数,预测其半衰期(t1/2)和活化能.结果:葛根素在不同环境条件下的降解反应均遵循一级动力学规律,在中性pH环境中最为稳定,碱性条件下较容易降解,离子强度和初始浓度对其降解影响不大.结论:葛根素在水溶液中较为稳定,仅在强酸和强碱环境下发生降解,呈现pH和温度依赖性,提示葛根素的保存适宜在低温中性条件下,避免极端的pH和高温条件.%Objective:To investigate the degradation kinetic characteristics of puerarin in aqueous solution under different pH and temperatures.Methods:The concentration change of puerarin in aqueous solution was determined by HPLC under different pH and temperatures.Based on the chemical reaction kinetic,the parameters of degradation kinetics were calculated.The activation energy and half-life were evaluated.Results:The results indicated that thermal degradation kinetic model of puerarin in aqueous solution follows first-order degradation kinetic process.Conclusion:Puerarin was more stable in neutral aqueous solution and low temperature condition.The degradation of puerarin can be observed in both strong acid and strong alkali conditions.