分散液相微萃取-气相色谱/质谱测定熏制食品中的多环芳烃

摘要

将加速溶剂萃取（ASE）与分散液相微萃取（DLLME）相结合，并与气相色谱，质谱法联用，建立一种快速、灵敏的测定熏制食品中多环芳烃的方法。对熏制食品中的多环芳烃先进行ASE萃取；再利用DLLME进行富集浓缩，最后取沉积相进行GC—MS分析。在优化的条件下，15种多环芳烃呈良好线性关系，相关系数为0．9900以上，检出限为0．44-214．07μg/mL，相对标准偏差为4．28％-14．56％。栓出熏火腿样品中多环芳烃的总含量为0．58μg/g，熏肉样品多环芳烃的总含量为0．70μg/g，2种样品中苯并【a】芘均未被检出。该方法简便、快速、灵敏，适用于熏制食品中多环芳烃的检测分析。%A new method based on accelerated solvent extraction (ASE)and dispersive liquid-liquid microextraction (DLLME)with gas chromatography-mass spectrometry (GC-MS)was proposed for the analysis of polycyclic aromatic hydrocarbons (PAHs)in smoked food. The smoked food was firstly extracted by ASE ,then concentrated by DLLME,the sedimentary phase of DLLME was analyzed by GC-MS. Under the optimized conditions,linear response data were well obtained for PAHs with correlation coefficients over 0.990 0, detection bruits were 0.44-214.07 pg/mL, and relative standard deviations (RSD)were between 4.28%- 14.56%. The total content of PAHs was 0.58 μg,/g in the smoked ham and 0.70 μg/g in the smoked bacon, benzo (a)pyrene was not detected in the both sample. The developed method is simple, rapid, sensitive, suitable for the determination of PAHs in smoked food.