怀地黄的茎尖组织培养和快繁技术研究

摘要

The paper aimed to study thevirus-free tissue culture and the rapid propagation technology.With Rehmannia glutinosa Libosch shoot tip as explants,MS was used as the basic medium with different concentration of NAA,IAA,2,4-D and 6-BA to configure different media,the tests of differentiation and induction,subculture and proliferation as well as rooting culture on Rehmannia glutinosa Libosch were carried out and the test-tube plantlets were hardened and transplanted.In the experiment,the media of detoxification and induction,proliferation and rooting were obtained as well as the external environmental conditions for the plant growth and the matching technology.%研究怀地黄组织培养与快繁技术。以怀地黄茎尖部分为外植体，在以MS作为基本培养基的基础上，添加不同浓度的NAA、IAA、2，4-D、6-BA，配制不同浓度的培养基，对怀地黄的茎尖进行分化与诱导、继代与增殖及生根培养试验，并对试管苗进行炼苗移栽。获得了怀地黄茎尖培养脱毒与诱导、增殖和生根的培养基，以及生长所需要的外部条件和配套技术。