论著军用功能食品NB-5在心理应激介导氧化应激中的作用研究

摘要

目的：研究心理应激中的氧化损伤效应，并探索新型军用功能食品NB-5在心理应激导致氧化应激中的保护作用。方法应用剪除大鼠胡须的方法建立心理应激实验动物模型，通过对脂质过氧化产物丙二醛（ MDA）以及蛋白质羰基含量的检测研究心理应激下大鼠不同脏器组织的氧化损伤程度；将NB-5加工成动物饲料喂食大鼠，4周后剪除胡须造成心理应激，并通过对以上指标的检测探索NB-5的防护效果。结果与结论大鼠剪除胡须后产生情绪焦虑导致包括大脑皮质、心、肝、脾和肾组织膜脂和蛋白质氧化损伤，过氧化氢酶活性显著降低，在该模型中NB-5表现出良好的自由基清除功能，并且能够显著提高过氧化氢酶的活性。新形势下，军用功能食品NB-5可缓解部队官兵在应对突发事件时因心理应激而造成的身体伤害，提高部队官兵的战斗力。%Objective To explore the protective effects of a new military functional food NB-5 on psychological stress-induced oxidative stress .Methods Rat whiskers were completely removed to induce the oxidative stress , and the concen-trations of MDA and protein carbonyl in various organs were detected to study the damage to membrane lipid and protein . Rats were fed with NB-5 for 4 weeks, and the oxidative stress was induced by whisker cutting .Biochemical marks men-tioned above were detected to explore the protective effects of NB-5.Results and Conclusion Lipid and protein peroxida-tion occurred in the brain , heart, liver, spleen and kidney after whisker removal due to emotional stress , while the catalase ( CAT) activity decreased significantly in these organs except the spleen .In this experiment model , NB-5 showed a good free radical scavenging activity to reduce the lipid and protein peroxidation among whisker -cutting rats fed with NB-5 in ad-vance.So NB-5 can serve as a good food for soldiers in case of emergency incidents .