二十二碳六烯酸-磷脂对小鼠睡眠的改善作用

摘要

Objective To study the sleep improvement function of DHA-PC.Methods The mice were randomly divid-ed into control, vehicle, DHA+Lecithin (60+200 mg/kg) and DHA-PC(50,100,200 mg/kg) groups.Ten mice were enrolled in each group .The mice of control were administered with normal food , the vehicle group was orally given normal saline at the dosage of 0.2 ml/10 g, while both DHA-PC and DHA+Lecithin were orally given corresponding drugs at the dosage of 0.2 ml/10 g.All the groups were treated for 30 days except control group .The direct sleep-inducing test, the test of lengthening sleep time induced by pentobarbital sodium , the test of pentobarbital sodium subthreshold-hypnosis and the test of barbital sodium sleep latency were conducted to observe the inductive effect of DHA -PC.Results Neither the effect on mice body mass nor directly-induced sleep was observed .DHA-PC (50,100, and 200 mg/kg) could prolong sleep time to (56.2 ±13.7),(57.9 ±25.4) and(64.1 ±18.4) min, respectively,compared to vehicle(32.9 ±10.8)min (P<0.05).DHA+Lecithin could not prolong sleep time (38.6 ±11.7)min compared to (32.9 ±10.8)min of vehicle.There was significant difference compared with DHA-PC at the dosage of 200 mg/kg (64.1 ±18.4)min (P<0.05).DHA-PC (200 mg/kg) enhanced pentobarbital sodium subthreshold-hypnosis (70%) compared to vehicle (10%) (P<0.05),so did DHA+Lecithin (60%) compared to vehicle (10%) (P<0.05).Both DHA-PC (200 mg/kg)[(22.9 ±4.1)min ] and DHA+Lecithin [(19.5 ±2.7) min ]could shorten sleep latency compared to vehicle (31.3 ±6.9) min(P<0.01), and the sleep latency of DHA +Lecithin (19.5 ±2.7) min was shorter than that of DHA-PC(50,100 mg/kg).Conclusion DHA-PC has some effect some sleep improvement in mice .%目的：研究二十二碳六烯酸-磷脂（ DHA-PC ）对小鼠睡眠的改善作用。方法小鼠随机分为正常对照组、溶剂对照组、DHA鱼油＋卵磷脂联合用药（DHA＋Lecithin）（60＋200）mg/kg（阳性对照）、DHA-PC 50、100、200 mg/kg组，每组10只。正常对照组小鼠自由饮食饮水，溶剂对照组小鼠按0．2 ml/10 g的体积灌胃给予生理盐水， DHA＋Lecithin（60＋200）mg/kg组和DHA-PC 50、100、200 mg/kg组小鼠均按0．2 ml/10 g的体积灌胃给药，1次/d，连续30 d。通过测定小鼠直接睡眠时间、戊巴比妥钠诱导睡眠时间、阈下剂量睡眠发生率及巴比妥钠诱导睡眠潜伏时间，观察DHA-PC对小鼠睡眠的作用。结果3种剂量的DHA-PC对小鼠体质量均无影响。直接睡眠实验结果表明，3种剂量的DHA-PC对小鼠直接睡眠无影响。戊巴比妥钠诱导睡眠实验结果表明， DHA-PC 50、100、200 mg/kg组小鼠戊巴比妥钠诱导睡眠时间分别为（56．2±13．7），（57．9±25．4）及（64．1±18．4）min，明显高于溶剂对照组（32．9±10．8） min （ P ＜0．05）；DHA ＋Lecithin （60＋200） mg/kg 组小鼠的戊巴比妥钠睡眠时间为（38．6±11．7）min，高于溶剂对照组小鼠（32．9±10．8）min，但无明显差异；DHA-PC 200mg/kg组明显高于DHA＋Lecithin （60＋200）mg/kg组（P＜0．05）。协同戊巴比妥钠阈下剂量催眠实验结果表明，DHA-PC 200 mg/kg组小鼠协同戊巴比妥钠阈下剂量催眠入睡率（70％）明显高于溶剂对照组（10％）（P＜0．05）；DHA＋Lecithin （60＋200） mg/kg组小鼠协同戊巴比妥钠阈下剂量催眠入睡率（60％）明显高于溶剂对照组（10％）（ P＜0．05）。巴比妥钠诱导睡眠潜伏期实验结果表明，DHA-PC 200 mg/kg和DHA＋Lecithin （60＋200） mg/kg组小鼠巴比妥钠诱导睡眠潜伏时间分别为（22．9±4．1）和（19．5±2．7）min，明显低于溶剂对照组（31．3±6．9）min（P＜0．01），且DHA＋Leci-thin（60＋200）mg/kg组小鼠巴比妥钠诱导睡眠潜伏时间为（19．5±2．7）min，明显低于DHA-PC 50和100 mg/kg组，二者分别为（27．3±2．4）和（26．9±4．2）min。结论 DHA-PC具有改善小鼠睡眠的作用。