发光细菌在不同生理介质中增殖活性的可视化研究

摘要

目的 探讨两种血小板污染常见细菌,即大肠埃希菌(大肠杆菌,ECO)和铜绿假单胞菌(PAE)在单采血小板、血浆以及磷酸盐缓冲液(phosphate buffered saline,PBS)中的菌落数与发光强度之间的关系,并探究其在不同生物流体介质中的生长增殖情况.方法 首先构建了上述两种发光细菌,用小动物活体成像仪观察其在不同介质中菌落数与发光强度之间的相关性,并取值进行相关性分析.然后观察其在不同介质中不同时间点的发光强度,绘制生长曲线.结果 在血小板、血浆、PBS中大肠杆菌和铜绿假单胞菌的菌落数与发光强度符合线性正相关,其中,血小板明显地抑制了两种细菌的增殖,血浆对其生长也有一定的抑制作用,但不如血小板抑菌作用强.结论对于构建的上述两种发光细菌,发光强度可代表细菌生长增殖情况.血小板对两种细菌的生长具有明显的抑制作用,血浆也具有一定的抑菌作用,但其作用不如血小板强.%Objective To investigate the relationship between the number of colonies and the luminous intensity of two kinds of platelets contamination bacteria,Escherichia coli(ECO)and Pseudomonas aeruginosa(PAE)in single platelets, plasma and phosphate buffered salines(PBS), and to explore their growth and proliferation in different biological fluid media.Methods The two luminescent bacteria were constructed before the correlations between the number of colonies and the luminous intensity in different media in vitro were observed with a small animal bioluminescence imaging instrument, and correlation analysis was carried out.By recording the luminous intensity at serial time points in different media, a growth curve was drown to reflect the proliferation of bacteria.Results There was a significant positive correlation between the number of colonies and luminous intensity of ECO and PAE in single platelets,plasma and PBS.The larger the number of colonies was,the stronger the luminous intensity was.The results indicated that single platelets significantly inhibited the proliferation of ECO and PAE,and that plasma also had some inhibitory effect,but not so strong.Conclusion For the two luminous bacteria,luminous intensity can represent the growth and proliferation of bacteria.Single platelets have obvious inhibitory effect on the growth of ECO and PAE, and the plasma also has some inhibitory effect, but the effect is not so strong as that of single platelets.