线粒体呼吸链在高压氧诱导HUVEC活性氧产生中的作用

摘要

Objective To investigate the role of mitochondrial respiratory chain (MRC) in induction of reactive oxygen species (ROS) by hyperbaric oxygen (HBO).Methods Superoxide anion (O·-2) specific fluorescence probe DHE and mitochondrial O·-2 probe Mito-SOX were used to label ROS in human umbilical vein endothelium cells(HUVEC) microscopically after HBO exposure.Results After HBO exposure, O·-2 increased (31±8)% and (137±19) % in whole cells and in mitochondria, respectively (P<0.01).These increments were suppressed by MRC complex Ⅱ inhibitor TTFA for (55±11)% in whole cells (P<0.05)and (61±8) % in mitochondria(P<0.01).Conclusion MRC may be the main source of ROS induced by HBO in HUVECs.%目的 探讨线粒体呼吸链在高压氧(hyperbaric oxygen,HBO)诱导细胞活性氧产生中的作用.方法 采用特异性荧光探针DHE和Mito-SOX分别标记全细胞和线粒体内超氧阴离子(O·-2),以荧光显微技术检测HBO暴露后人脐静脉内皮细胞(human umbilical vein endothelium cells,HUVEC)内O·-2的生成.结果 HBO暴露后全细胞O·-2升高(31±8)%(P<0.01),线粒体内O·-2升高(137±19)%(P<0.01);给予呼吸链复合体Ⅱ抑制剂TTFA后,两者O·-2的增长分别被抑制(55±11)%(P<0.05)和(61± 8)%(P<0.01).结论 HBO主要通过线粒体呼吸链增加HUVEC活性氧生成.