[目的]应用一种昆虫细胞培养基作为基础成分培养巴尔通体(Bartonella species),建立一种操作方便、高效稳定的巴尔通体液体培养方法.[方法]昆虫细胞培养基中添加10％胎牛血清,以此为基础培养液分别添加蔗糖和谷氨酰胺,比较这两种成分对汉赛巴尔通体(B.henselae)和五日热巴尔通体(B.quintana)生长的影响并观察其他10种巴尔通体在简化后的培养液中的生长特性.[结果]添加蔗糖和谷氨酰胺不会明显促进巴尔通体的生长,10种巴尔通体在简化后的培养液中均生长良好.不同种巴尔通体生长曲线不同,汉赛巴尔通体和五日热巴尔通体的世代时间分别为5.2 h和4.3 h,生长速度快于固体培养.[结论]以昆虫细胞培养基作为基础成分的培养液适于巴尔通体液体培养,特别是对一些更难培养的巴尔通体提供了一种较好的培养方法.%[Objective] We tested an insect cells-based liquid medium and its utility for the easy-to-maintain, fast and reliable system for cultivation of different Bartonella.[Methods] Media composition and growth conditions were optimized using B.henselae and B.quintana.The growth curves of the other ten Bartonella species were determined using the optimized liquid medium.[Results] Glutamine and sucrose supplements did not affect significantly the growth rate of these bacteria.A generation time of 5.2 h for B.henselae and 4.3 h for B.quintana was estimated during the exponential phase of the growth curve occurred between days 1 and 2 after inoculation.The twelve medical and veterinary important Bartonella species grew well under conditions established and each exhibited unique growth characteristics.[Conclusion] This liquid growth medium may provide an advantage over conventional direct blood agar plating for analyzing Bartonella properties including antibiotic susceptibility and resistance and pathogeniciry.
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