鸡血藤总黄酮对坐骨神经损伤大鼠机械痛敏影响的实验研究

摘要

Objective To study the effects of total flavonoids in Net Cliffbean on the mechanical allodynia and glutamate contents in spinal of chronic constriction injury of the sciatic(CCI) rats.Methods 40 rats were randomly divided into 4 groups: control group, sham operation group, CCI group and Net Cliffbean treatment group, with NomralSaline or drugs, once a day, for 4 weeks. Mechanical withdrawal threshold (MWT) was measured as the sign of mechanical allodynia for 3, 7, 10, 14, 21, 28 day. The lumbar segment of the spinal cord was removed for determination of glutamate contents by high pressure liquid chromatography (HPLC) for 14, 28 day.Results Total lfavonoids in Net Cliffbean signiifcantly increased the mechanical withdrawal threshold (MWT) compared with model group (P<0.05 orP<0.01), and obviously decreased the content of glutamate in the spinal cord (P<0.01).Conclusion Total lfavonoids in Net Cliffbean can relieve the mechanical allodynia in neuropathic pain rats, its mechanism maybe reduced glutamate content in the spinal cord.%目的：研究鸡血藤总黄酮对坐骨神经损伤（chronic constriction injury of the sciatic，CCI）大鼠机械痛敏和脊髓谷氨酸含量的影响。方法40只大鼠随机均分为4组：对照组（腹腔注射生理盐水，不手术）、假手术组（分离坐骨神经但不结扎+腹腔注射生理盐水）、模型组（坐骨神经结扎+腹腔注射生理盐水）、鸡血藤总黄酮处理组（坐骨神经结扎+腹腔注射鸡血藤总黄酮20 g/kg），连续给药4周。运用机械缩足反射阈值检测术后3、7、10、14、21、28 d大鼠机械痛敏。运用高效液相法测定大鼠术后14、28 d脊髓背角中谷氨酸的含量。结果与模型组比较，处理组术后第10、14、21、28天的机械缩足反射阈值明显升高（P＜0.05,或P＜0.01）；在第14天及第28天观测到鸡血藤总黄酮可显著降低大鼠脊髓背角谷氨酸的含量（P＜0.01）。结论鸡血藤总黄酮可减轻神经病理性痛大鼠的机械痛敏，其机制可能与降低脊髓背角谷氨酸的含量有关。