重组IL-12基因转染对抗淋巴瘤Raji细胞增殖、凋亡的影响

摘要

目的：通过观察IL-12重组腺病毒载体对细胞毒性T淋巴细胞杀伤淋巴瘤Raji细胞增殖、凋亡的影响，为进一步基因治疗人Burkitt淋巴瘤提供实验依据。方法：培养淋巴瘤特异性CTL细胞，观察其对重组腺病毒载体转染Raji细胞的杀伤作用，MTT法检测Raji细胞的凋亡率，LDH释放实验检测CTL细胞毒作用。结果：各组均能抑制Raji细胞的生长。其中A组（Ad IL-12-Raji细胞与CTL细胞混合培养组）细胞凋亡率（49.1±7.2）%与CTL细胞的杀伤率（93.52±3.4）%均明显高于B、C两组。结论：经IL-12基因转染修饰的肿瘤细胞，可以增强CTL细胞的杀伤能力。%Objective:In order to understand the resistance mechanism and further overcome the Burkitt Lymphoma Raji Cells, we study the proliferation and apoptosis effect of adenovirus-mediated interleukin-12 on the Raji Cells. Methods:High ratio of activated CTL,which can kill Raji cells were gotten by DC. Cultered Raji Cells were infected with the recombinant adenovirus vector haboring IL-12 gene to establish Ad-IL12-Raji. The proliferation and apoptosis of Raji Cells were detected by MTT . The cytotoxicity of CTL were detected by lactate dehydrogenase(LDH).Results:The proliferation and apoptosis of Raji cell can be restrained in all groups. The proliferation and apoptosis(49.1±7.2)%,cytotoxicity of CTL(93.52±3.4)% in groups A can be significantly up-ragulated than the others. Conclusions:The cells with IL-12 gene transduction can inhibit the proliferation and induce apoptosis of Raji by induce the cytotoxicity of CTL.