A DNA Construct that Alters the expression of an endogenous g-csf Gene in a Mammalian Cell in Integration into the cell Genome through homologous derecombinacion,Homologamente Cell stably transfected with a recombinant DNA Construct, a method of altering the expression

摘要

A DNA Construct that Alters the expression of an endogenous g-csf Gene in a Mammalian Cell in Integration into the cell Genome through homologous derecombinacion,The Construct includes: (i) a selected Target Sequence that contains at least 20 contiguous nucleotides of SEQ idno.: 5 and (ii) a regulatory sequence of transcription, Cell homologamente stably transfected with a recombinant DNA construct.A method of altering the endogenous g-csf gene expression in a Mammalian Cell, this cell for use in the manufacture of a Medicament.Method of production of g-csf and an isolated Nucleic Acid Molecule.