A method for Controlling the expression of a structural Gene in a cell, DNA constructs for use in said method capable of altering the expressionof The Gene Coding of thrombopoietin.DNase or interferon beta, isolated DNA Molecule, homologamente recombinant cells, Methods for producing those

摘要

A method for controlling gene expression of cell structure is described, including (a) providing various stages of anti tank structure, including directional insertion sequence, regulation sequence and junction position; (b) An intervening DNA sequence is established between the regulatory sequence and the structural gene, which combines the structural sequence with the structural gene of the corresponding recombinant cell, so that it is in a pre screened position with the gene structure, so as to generate the corresponding recombinant cell;The inserted building adopts a configuration, according to which the regulatory performance is separated from the structural gene, and the length of adn intervenor is pre screened, while the donated bonding site is placed in a way, in the connection after the reprint, RNA cognition and main(c) Control the structural expression of the selected interfering DNA of level (b). The insertion structure in the design at least includes: (a) directional sequence; (b) regulatory sequence; (c) foreign object; and (d) donor connection point. This structure can be repurchased by endogenous cell sequences to generate new genes, which can be imported into cells to generate corresponding recombinant cells. Cells produce a thromboid cellDNase I or interferon Beta. The invention further relates to methods of production of pharmaceutically useful preparations containing thrombopoietin, DNase (I) or beta interferon from cells that are exclusively recombinant and methods of therapy.