白介素-8在原发性痛风性关节炎患者外周血的达及意义

摘要

Objective To analyze the expression and clinical significance of IL-8 in peripheral blood in patients with gouty arthritis (GA).Methods The peripheral blood were collected from 43 patients with acute gouty arthritis (AGA),43 patients with non-acute gouty arthritis (NAGA) and 43 healthy controls (HC).The expression levels of IL-8 mRNA and protein in peripheral blood mononuclear cells (PBMCs) were detected by real-time fluorescent quantitative polymerase chain reaction and Western blot.The protien expressions of IL-8 were measured by enzyme linked immunosorbent assay (ELISA) in plasma.Results The expression of IL-8 mRNA in PBMCs of the AGA group was significantly higher than that of the IGA and HC groups (all P＜0.01),and IGA group higher than HC groups (P＜0.01).Compared with IGA and HC groups,the protein expression of IL-8 significantly increased in PBMCs of the AGA group,and the HC group was the lowest.There were no statistical differences between IGA and HC groups.The expression of IL-8 was significantly higher in plasma of the AGA group compared with the IGA and HC groups (all P＜0.01),and IGA group significantly higher than HC groups (P＜0.05).Conclusion The expression of IL-8 changes significantly in GA patients in comparison with HC,which implies that IL-8 may have an important role in progression of GA.%目的 分析白介素-8(IL-8)在痛风性关节炎(GA)患者外周血中的表达变化,探讨其临床意义.方法 选择急性痛风性关节炎(AGA组)、非急性痛风性关节炎间歇期(NAGA组)和健康体检者(HC组)各43例.利用RT-PCR和Western blot方法分别检测外周血单个核细胞(PBMCs)中IL-8mRNA和蛋白表达,再用ELISA法检测各组血浆IL-8水平.结果 IL-8 mRNA在AGA组PBMCs中的表达高于NAGA组和HC组(均P＜0.01),在NAGA组的表达高于HC组(P＜0.01).PBMCs中的IL-8蛋白水平在AGA组高于NAGA组和HC组(均P＜0.01),且HC组最低,但NAGA组和HC组比较差异无统计学意义(P＞0.05).血浆中IL-8水平在AGA组和NAGA组显著高于HC组(均P＜0.01),NAGA组显著高于HC组(P＜0.05).结论 IL-8在GA患者的外周血中异常表达,提示IL-8在GA的病理机制中可能发挥重要的作用.