逐瘀通脉胶囊对脑缺血再灌注大鼠学习记忆能力的影响

摘要

目的:观察逐瘀通脉胶囊对反复夹闭颈总动脉(CCA)脑缺血再灌注大鼠学习记忆能力的影响.方法:SD大鼠70只,随机取10只为假手术组,不阻断CCA,不注射硝普钠;另60只反复夹闭CCA,同时给予硝普钠(25 mg/kg)建立脑缺血再灌注损伤大鼠学习记忆障碍模型,造模成功后分为6组:模型组、维脑路通组(100 mg/kg)、步长脑心通组(800 mg/kg)、逐瘀通脉高、中、低剂量组(200、100、50 mg/kg).给药14 d后,利用Morris水迷宫进行定位航行实验、空间探索测试,评价动物学习记忆能力.结果:与模型组比较,逐瘀通脉高剂量组(200 mg/kg)大鼠在定位航行试验中找到平台次数明显增加,逃离潜伏期时间以及寻找平台游经总路程明显减少(P＜0.01),在第三象限停留时间显著延长(P＜0.01).空间探索试验撤除平台后,逐瘀通脉高、中剂量组(200 mg/kg、100 mg/kg)大鼠在有效区域停留的时间和游经距离显著延长(P＜0.01),经过虚拟平台的次数显著增加(P＜0.01);100 mg/kg剂量组动物经过有效区域的次数显著增加(P＜0.01),200 mg/kg剂量组大鼠在第一象限停留时间显著缩短,第三象限停留时间和距离显著延长(P＜0.01).结论:逐瘀通脉胶囊对脑缺血再灌注损伤大鼠定位记忆能力和空间探索能力的下降有明显改善作用.%Objective:To observe the effects of Zhuyu Tongmai capsule on learning and memory abilities of rats with cerebral ischemia.Methods:Seventy SD rats,ten rats were randomly selected as sham operation group,CCA was not blocked and sodium nitroprusside was not injected.Another 60 rats were duplicated by repeated clamping of bilateral common carotid artery combined with sodium nitroprusside(25 mg/kg) to establish cerebral ischemia rats model,the rats after success established were divided into 6 groups:model group,Venorruton group(100 mg/kg),Naoxintong group(800 mg/kg),Zhuyu Tongmai high,middle and low dosage group(200,100,50 mg/kg).After 14 days,Morris water maze was used to place navigation test,spatial probe test,and evaluate the animal learning and memory ability.Results:Compared with model group in place navigation test,Zhuyu Tongmai high dosage group (200 mg/kg) increased the times of rats finding the platform,and reduced the escape latency,looking for the platform to travel the distance(P＜0.01),and extended the stay in the third quadrant pool.In spatial probe test,Zhuyu tongmai high and middle dosage group (200,100 mg/kg) extended the stay in the effective area and travel distance(P ＜0.01),and rised the times of crossing the virtual platform(P＜0.01).Zhuyu Tongmai group(100 mg/kg) increased the times of crossing the effective area(P＜0.01),extended the stay distance in the third quadrant pool (P＜0.01).Conclusion:Zhuyu Tongmai capsule is obviously improve the spatial memory ability and active exploration ability of rats with cerebral ischemia.