MiR-340经组织型纤溶酶原激活剂调控施万细胞的纤溶能力

摘要

目的：探讨microRNA-340（miR-340）对施万细胞纤溶能力的调控作用及作用的靶基因。方法：采用溶圈实验来检测miR-340对施万细胞纤溶能力的影响，用荧光素酶双报告基因系统确定 miR-340与组织型纤溶酶原激活剂的靶向关系，用实时定量聚合酶链反应检测坐骨神经夹伤后组织型纤溶酶原激活剂mRNA和miR-340的表达变化。结果：miR-340能够抑制施万细胞的纤溶能力，并直接靶向作用于组织型纤溶酶原激活剂的3'UTR，坐骨神经夹伤后组织型纤溶酶原激活剂mRNA与miR-340的表达呈负相关性。结论：miR-340通过直接靶向组织型纤溶酶原激活剂的3'UTR，从而下调靶基因组织型纤溶酶原激活剂的表达抑制施万细胞的纤溶能力。%Objective: To investigate the regulatory role of miR-340 on the fibrinolytic capacity of Schwann cells and the target gene of miR-340. Methods: Fibrinolysis assay was used to examine the influence of miR-340 on the fibri-nolytic capacity of Schwann cells. Dual luciferase assay system was used to determine whether miR-340 targets the 3'UTR of tPA. Real-time PCR was used to examine the expression of tPA mRNA and miR-340 follwoing sciatic nerve injury. Results: miR-340 inhibits the fibrinolytic activity of Schwann cells and targets the 3'UTR of tPA directly. The expression of tPA mRNA was negatively correlated with that of miR-340. Conclusion: This study reveals that miR-340 inhibits the fibrinolytic activity of Schwann cells by targeting tPA.