目的：使用内皮祖细胞再内皮化胰腺脱细胞支架，并初步评价其在宿主体内生成血管的能力。方法：采用灌注法制备结构完整的全胰腺脱细胞支架；并提取大鼠骨髓单个核细胞，诱导分化为内皮祖细胞，进行原代培养；将培养完成的内皮种植于支架内，并行体内移植。结果：在移植后第10天，实验组中包含红细胞的血管管腔数为（43±6）cm2，而对照组为（8±3）cm2；在移植后第20天，实验组中包含红细胞的血管管腔数为（62±9）cm2，而对照组为（16±5） cm2，此结果具有统计学意义（P<0.05，n=6）。种植内皮祖细胞后，支架在体内能更快更多的生成新生血管，与对照组比较差异有统计学意义（P<0.05）。结论：再血管化的支架在体内移植后，与对照组相比，可以更快的与宿主循环系统形成吻合。%Objective: To recellularize the Pancreatic De-Cellularized Scaffold with endothelial progenitor cells (EPCs) and evaluate its potential function of endothelialization in vivo. Methods: Decellularized pancreatic scaffolds were obtained by perfusing method. Bone marrow mononuclear cells (BMMNCs) were isolated, cultured and induced into EPCs. The scaffolds were implanted in vivo after recellularization by EPCs. Results: Quantitative results show that the density of new blood vessels in the endothelialized scaffold was significantly higher than that in the control group. Conclusion:Compared with the control group, the endothelialized scaffold can be connected with the host circulatory system faster.
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