目的 探讨Aurora-B、Ki-67在骨肉瘤中的表达及与远处转移的关系.方法 收集2002年1月-2009年3月手术切除的60例骨肉瘤组织石蜡标本,采用免疫组织化学方法检测Aurora-B和Ki-67的表达,以同期21例骨软骨瘤组织作为对照.结果 Aurora-B蛋白在骨肉瘤组织中的阳性率(53.3％)明显高于骨软骨瘤(14.3％,P＜0.05),且在伴远处转移的骨肉瘤组织中(78.6％)明显高于无远处转移者(45.7％,P＜0.05).Ki-67增殖指数在骨肉瘤组织中为35.1％±15.2％,明显高于骨软骨瘤组织(2.1％±0.9％,P＜0.01),且在伴远处转移的骨肉瘤组织中(48.3％％±20.1％)明显高于不伴远处转移者(21.2％±10.2％,P＜0.05).相关分析显示,骨肉瘤组织中Aurora-B和Ki-67蛋白表达呈显著正相关(r=0.885,P＜0.01).结论 Aurora-B和Ki-67蛋白可能与骨肉瘤的发生及远处转移有关,有望成为骨肉瘤新的诊断及分子治疗靶点.%Objective To investigate the correlation of the expressions of Aurora-B and Ki-67 protein in osteosarcoma, and the relationship of the expressions with distant metastasis. Methods Sixty formalin-fixed, paraffin-embedded surgical specimens of osteosarcoma obtained from Jan. 2002 to Mar. 2009 were used to observe the expressions of Aurora-B and Ki-67 by immunohistochemical staining, and 21 specimens of osteochondroma obtained at the same period were similarly studied to serve as control. Results The positive rate of Aurora-B was significantly higher in osteosarcoma (53. 3%) compared with osteochondroma (23. 8%, P<0. 05) , and was markedly higher in osteosarcoma with distant metastasis (78. 6%) than in osteosarcoma without distant metastasis (45. 7%, P<0. 05). The Ki-67 proliferation index was significantly higher in osteosarcoma (35. 1%±15. 2%) than in osteochondroma (2. L%±0. 9%, P<0.01), and was markedly higher in osteosarcoma with distant metastasis (48. 3%+20. 1%) than in osteosarcoma without distant metastasis (21. 2% + 10. 2%, P<0. 05). Correlation analysis revealed that the Aurora-B positive rate was positively correlated with Ki-67 proliferation index (r=0.885, P<0.01) in osteosarcoma. Conclusion Aurora-B and Ki-67 protein may be involved in the development, progression and metastasis of osteosarcoma, and is expected to serve as a new diagnostic and therapeutic target for osteosarcoma.
展开▼
