Expression of KiSS-1 in epithelial ovarian cancer and its role in metastasis

摘要

Objectives To study expression of KiSS-1 and its role in migration and invasion of ovarian cancer(OC). Methods Expression of KiSS-1 was detected in tissue of 46 cases of OC and 17 cases of benign ovarian neoplasm by immunohistochemistry examination. Human OC cell line HO8910 was transfected by pcDNA3-KiSS-1 vector. The cell proliferation and invasion properties were detected by RT-RCR, MTT, clone formation rate and Boyden Chamber invasion assay. Results (1) Immunostaining showed that expression of KiSS-1protein was significantly higher in OC than that in benign ovarian tumor (P<0.05). (2) KiSS-1 expression was significantly higher in cases of advanced stage and with lymphatic metastasis (P<0.05). KiSS-1 expression was significantly lower in clear cell cancer compared with other histologic types (P<0.05). (3)KiSS-1 gene was successfully integrated into the genomic DNA of ovarian cancer cell line HO8910. Boyden Chamber invasion assay revealed that the number of cells invading through the Matrigel filter was significantly decreased in the transfected group compared with the non-transfected. No differences were observed in cell proliferation between the two groups. Conclusion There was over expression of KiSS-1 in OC compared with that in benign ovarian tumor. The KiSS-1 gene could suppress HO8910 invasion in vitro. To elucidate the contradiction effects of metastasis suppressor genes KiSS-1 in vivo and in vitro needs deeper research. Ovarian cancer is the most lethal gynecologic malignancy. It classically spreads by locoregional peritoneal dissemination and metastasis. KiSS-1 gene was discovered in metastasis-suppressed melanoma and known to strongly respond to tumor cell invasion suppression. The transfer of its cDNA clone into metastatic melanoma significantly suppressed metastasis without affecting tumorigenicity. KiSS-1 maps to chromosome 1q32, is one of genes regulated by chromosome 6. Recently, products of the KiSS-1 gene (kisspeptins) have been identified to inhibit metastasis in various tumors, via an unknown mechanism, perhaps via binding to the G-protein-coupled receptor. We detected the expression of mRNA and product in a comprehensive collection of ovarian cancer.