姜黄素对人宫颈癌细胞株Hela细胞增殖的作用的研究以及p53表达的影响

摘要

目的：主要探讨姜黄素对人类宫颈癌Hela细胞中增殖作用的影响，以及细胞p53蛋白表达的影响。方法：本文通过不同浓度姜黄素干预人宫颈癌Hela细胞，采用MTT法检测药物对Hela细胞的增殖抑制率；RT-PCR和Western blot检测细胞凋亡相关蛋白p53表达。结果：MTT发现姜黄素对人宫颈癌Hela细胞有一定的抑制作用，并呈时间-剂量依赖性；RT-PCR结果显示p53mRNA的表达增加；western boltting显示p53蛋白的表达增加。结论：姜黄素可一直Hela细胞增殖，其机制可能是通过增加p53基因的表达而实现。%Objective:To observe the effect of cuicumin on proliferation of human cervical cancer Hela cells and expression of p53 protein. Method:In this paper,Hela cells were treated with curcumin at various concentrations for various hours,Then determined for proliferation activity by MTT method,for transcription levels ofβ-catenin and p53 mRNAs by RT-PCR,and for expressions ofβ-catenin and p53 protein by Western blot. Result:Curcumin showed dose-and time-dependent inhibitory effect on the proliferation of Hela cells.Meanwhile,curcumin increased the mRNA transcription and protein expression levels ofβ-catenin and p53.Conclusion:Curcumin inhibited the proliferation of Hela cells by a possible mechanism of increased the express of p53.