金葡菌法制备Wistar大鼠胫骨骨髓炎骨缺损模型

摘要

To observe the effect of making Wistar rats tibias osteomyelitic bone defect by putting a carbasus with Staphylococcus aureus in the bone defection.Method:30 Wistar tibias were divided into 3 groups randomly. The 1st group:control group,every tibia was putted bone defect. The 2nd group:every tibia was putted a carbasus with staphylococcus aureus(SA)in the bone defect. The 3rd group:every rat was used antibiotic therapy after the course of the 2nd group. The time of vulnus union,weight,rectal temperature,leuk-analysis,appearance of local tissue condition were compared,the tibias CR at 7 d,14 d for evaluating the course of osteomyelitic bone defect were observed.Result:Among the 3 group,there were remarkable differences at the the condition of vulnus union,weight,rectal temperature,leuk-analysis,appearance of local tissue condition,and tibias CR(P<0.05). Conclusion:Putting a carbasus with SA in the bone defection is a good way to make the modal of osteomyelitic bone defect.%目的：研究将蘸有金黄色葡萄球菌悬液的棉条植入Wistar大鼠胫骨骨缺损中制备骨髓炎骨缺损标本的方法。方法：将健康雄性Wistar大鼠30只按随机数字表法分为三组，每组10只20个后腿标本。第1组：在Wistar大鼠双侧胫骨近端制造骨缺损；第2组：将蘸有金黄色葡萄球菌悬液的棉条置入骨缺损中；第3组：应用抗生素治疗将蘸有金黄色葡萄球菌悬液的棉条置入骨缺损中的大鼠。比较各组大鼠创口愈合时间，并在7、14 d时观察大鼠体重、体温、伤口愈合、白细胞计数、胫骨标本及CR并进行比较。结果：三组在体重、体温、伤口愈合情况、白细胞计数、胫骨标本及CR表现积分上比较差异均有统计学意义（P<0.05）。结论：在胫骨骨缺损中植入蘸有金黄色葡萄球菌悬液的棉条是制备Wistar大鼠胫骨骨髓炎骨缺损的良好方法。