综合康复对大鼠受压坐骨神经微血管生成的影响

摘要

Objective:To observe the the effect of comprehensive rehabilitation on neural micro-angiogenesis in rat with sciatic nerve compression.Method:According to the rat model of sciatic nerve compression designed by Mackinnon,forty male SD rats were chosen and randomly divided into 4 groups(n=10):group A,group B,group C,and group D.After 2 weeks,compression were removed in all 4 groups,and no treatment was given in group A,rehabilitation training in group B,electroacupuncture in group C,electroacupuncture combined with rehabilitation training in group D.At 3 weeks after treatment,the sciatic nerve function index (SFI),and the expression of CD34,VEGF in the compression sciatic nerve of rats were measured.Result:The SFI recovery rate of groups B,C,and D were significantly higher than that of group A(P<0.05), and that of group D was significantly higher than that of groups B and C(P<0.05).The expression of CD34 and VEGF in the compression sciatic nerve of groups B,C,and D were significantly higher than that of group A(P<0.05),and that of group D was significantly higher than that of groups B and C(P<0.05).Conclusion:Comprehensive rehabilitation can improve neural micro-angiogenesis in rat with sciatic nerve compression,thereby it can promote the nerve regeneration and functional recovery.%目的：观察综合康复对大鼠受压坐骨神经微血管生成的影响。方法：参照经典Mackinnon大鼠坐骨神经卡压模型制作动物模型，40只大鼠完全随机分为A、B、C、D 4组，每组10只，2周后，A组（对照组）：仅去除卡压；B组（康复训练组）：去除卡压后行康复训练；C组（电针组）：去除卡压后行电针治疗；D组（康复训练+电针组）：去除卡压后行康复训练加电针治疗。术后第3周各组治疗结束。观察各组大鼠大体情况、坐骨神经功能指数（SFI）、卡压神经中段标本CD34、VEGF表达。结果：B、C、D组SFI恢复程度均大于A组（P<0.05）；D组SFI恢复程度明显大于B组与C组（P<0.05）。B、C、D组CD34、VEGF表达明显高于A组（P<0.05）；D组CD34、VEGF表达明显高于B组与C组（P<0.05）。结论：综合康复可以有效促进大鼠受压坐骨神经微血管生成，进而促进神经再生与功能恢复。