不同浓度、作用时间白花蛇舌草注射液对MG-63细胞凋亡效果

摘要

Objective:To explore the effect of spreading hedyotis herb injection of bone sarcoma MG-63 cells apoptosis induction.Method:With a certain concentration of ethanol extraction of spreading hedyotis herb active ingredients, the mixture concentration of 50, 100, 200, 400 μL/mL four different spreading hedyotis herb injection. And its injection into different concentrations of bone sarcoma MG-63 cells in vitro culture. Determined by MTT method was used to detect the different concentration of spreading hedyotis herb injection effect on bone sarcoma MG-63 cells, 12, 24 and 48 h after cell proliferation inhibition rate, and USES the quantitative RT-PCR detection of apoptosis related gene Bax mRNA expression. Cells and decodes the (FCM) spreading hedyotis herb injection of bone sarcoma with MG-63 cell apoptosis rate.Result:Determined by MTT method to detect the result shows that, with the spreading hedyotis herb injection concentration and action time, increasing bone sarcoma with MG-63 cell proliferation inhibition rate were significantly increased, when the concentration of spreading hedyotis herb injection up to 100 μL/mL, with the duration extension, Bax gene expression was significantly higher (P<0.05). Flow cytometry instrument detection showed that after spreading hedyotis herb injection treatment, bone sarcoma with MG-63 cells apoptosis rate with the increase of the concentration of spreading hedyotis herb injection and rising.Conclusion:Spreading hedyotis herb injection via increased Bax gene expression can induce bone sarcoma with MG-63 cell apoptosis, and with the increase of concentration of spreading hedyotis herb injection, bone sarcoma with MG-63 cells apoptosis rate also increased.%目的：探讨白花蛇舌草注射液对人骨肉瘤MG-63细胞凋亡的诱导作用。方法：采用一定浓度的乙醇提取白花蛇舌草有效成分，将其配制成50、100、200、400μL/mL 4种不同浓度的白花蛇舌草注射液，并注入到体外培养的人骨肉瘤MG-63细胞的培养液中。采用MTT法检测不同浓度白花蛇舌草注射液对人骨肉瘤MG-63细胞作用12、24、48 h后的细胞增殖抑制率，并采用RT-PCR半定量检测凋亡相关基因Bax的mRNA表达情况。同时拟用流式细胞仪（FCM）检测白花蛇舌草注射液对人骨肉瘤MG-63细胞凋亡率。结果：MTT法检测结果显示，随着白花蛇舌草注射液浓度和作用时间的不断增加，人骨肉瘤MG-63细胞增殖抑制率均明显增大；当白花蛇舌草注射液浓度达100μL/mL时，随着作用时间延长，Bax基因表达显著升高（P<0.05）。流式细胞仪检测显示，经白花蛇舌草注射液处理后，人骨肉瘤MG-63细胞凋亡率随白花蛇舌草注射液浓度的增加而不断升高。结论：白花蛇舌草注射液通过上调Bax基因表达能诱导人骨肉瘤MG-63细胞凋亡，且随着白花蛇舌草注射液浓度增加，人骨肉瘤MG-63细胞凋亡率亦不断增加。